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Article

Characterization of a Chinese hamster ovary cell mutant having a mutation in elongation factor-2.

PloS one - 5 Feb 2010

Gupta Pradeep K, Liu Shihui, Leppla Stephen H

Abstract excerpt

Retroviral insertional mutagenesis provides an effective forward genetic method for identifying genes involved in essential cellular pathways. A Chinese hamster ovary cell line mutant resistant to several bacterial ADP-ribosylating was obtained by this approach. The toxins used catalyze ADP-ribosylation of eukaryotic elongation factor 2 (eEF-2), block protein synthesis, and cause cell death. Strikingly, in the...

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Identifiers

PMID: 20140093
DOI: 10.1371/journal.pone.0009078
PMCID: PMC2816718

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Characterization of a Chinese hamster ovary cell mutant having a mutation in elongation factor-2.PMID 20140093Read ↗

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