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Article

New runaway-replication-plasmid cloning vectors and suppression of runaway replication by novobiocin.

Gene - 1 Jan 2000

Uhlin B E, Schweickart V, Clark A J

Abstract excerpt

Two new cloning vectors (pBEU28 and pBEU50) with temperature-controlled runaway-replication properties are described. pBEU28 is similar to aphA+ (KanR) plasmid pBEU2 but lacks a 1.8-kb duplication which is responsible for plasmid instability. pBEU50 is an analog of pBR313 and pBR322 in that it carries bla+(AmpR), which can be used for selection, and tet+(TetR) which can be inactivated by cloning at HindIII and...

Topics

Base Sequence
Chromosome Deletion
Chromosomes, Bacterial
Cloning, Molecular
DNA Replication
DNA Restriction Enzymes
Escherichia coli
Genotype
Mutation
Novobiocin
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 6307826
DOI: 10.1016/0378-1119(83)90110-5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-6307826-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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