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Article

New runaway-replication-plasmid cloning vectors and suppression of runaway replication by novobiocin.

Gene - 1 Jan 2000

Uhlin B E, Schweickart V, Clark A J

Abstract excerpt

Two new cloning vectors (pBEU28 and pBEU50) with temperature-controlled runaway-replication properties are described. pBEU28 is similar to aphA+ (KanR) plasmid pBEU2 but lacks a 1.8-kb duplication which is responsible for plasmid instability. pBEU50 is an analog of pBR313 and pBR322 in that it carries bla+(AmpR), which can be used for selection, and tet+(TetR) which can be inactivated by cloning at HindIII and...

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Identifiers

PMID: 6307826
DOI: 10.1016/0378-1119(83)90110-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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