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Article

Eclipse period during replication of plasmid R1: contributions from structural events and from the copy-number control system.

Molecular microbiology - 1 Oct 2003

Olsson Jan A, Berg Otto G, Dasgupta Santanu, Nordström Kurt

Abstract excerpt

The eclipse period (the time period during which a newly replicated plasmid copy is not available for a new replication) of plasmid R1 in Escherichia coli was determined with the classic Meselson-Stahl density-shift experiment. A mini-plasmid with the wild-type R1 replicon and a mutant with a thermo-inducible runaway-replication phenotype were used in this work. The eclipses of the chromosome and of the wild-type...

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PMID: 14507381
DOI: 10.1046/j.1365-2958.2003.03683.x

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