Article
[Selection of initiation replication mutants of IncP-9 plasmid pBS267].
Genetika - 1 Feb 2013
Sechenikov A A, Koval'chuk K V, Vasilenko S L, Titok M A
Abstract excerpt
A minireplicon containing the rep gene and oriV site of the gamma subgroup of the IncP-9 caprolactam pBS267 biodegradation plasmid was cloned for the first time. It was established that a minimized variant of pBS267 plasmid cannot be sustained in E. coli and is inherited in an unstable way in bacteria Pseudomonas. Using in vitro mutagenesis, mutant variants of the minireplicon were produced, characterized by an...
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