Article
Identification of a high-copy-number plasmid suppressor of a lethal phenotype caused by mutant DnaA protein which has decreased intrinsic ATPase activity.
Biological & pharmaceutical bulletin - 1 Sept 1999
Makise M, Sakamoto K, Tsuchiya T, Mizushima T
Abstract excerpt
The induction of a mutant DnaA protein (DnaA E204Q) with decreased intrinsic ATPase activity in cells causes a lethal phenotype. Based on our results that the decreased ATPase activity of DnaA E204Q is activated to the level of a wild-type protein in the presence of partially purified stimulating factors for DnaA ATPase, we tested the hypothesis that genes encoding the stimulating factors can be cloned as...
Topics
- Adenosine Triphosphatases
- Adenylate Cyclase Toxin
- Bacterial Proteins
- Cell Division
- Cloning, Molecular
- DNA Polymerase III
- DNA-Binding Proteins
- DNA-Directed DNA Polymerase
- Escherichia coli
- Escherichia coli Proteins
- Gene Dosage
- Genes, Bacterial
- Genes, Lethal