Article
Unique plasmids generated via pUC replicon mutagenesis in an error-prone thermophile derived from Geobacillus kaustophilus HTA426.
Applied and environmental microbiology - 1 Nov 2015
Kobayashi Jyumpei, Tanabiki Misaki, Doi Shohei, Kondo Akihiko, Ohshiro Takashi, Suzuki Hirokazu
Abstract excerpt
The plasmid pGKE75-catA138T, which comprises pUC18 and the catA138T gene encoding thermostable chloramphenicol acetyltransferase with an A138T amino acid replacement (CATA138T), serves as an Escherichia coli-Geobacillus kaustophilus shuttle plasmid that confers moderate chloramphenicol resistance on G. kaustophilus HTA426. The present study examined the thermoadaptation-directed mutagenesis of pGKE75-catA138T in...
Topics
- Acetyl Coenzyme A
- Chloramphenicol O-Acetyltransferase
- Chloramphenicol Resistance
- Escherichia coli
- Genetic Vectors
- Geobacillus
- Hot Temperature
- Mutagenesis
- Mutation
- Plasmids