Article
Characterization of an efficient gene cloning strategy for Aspergillus niger based on an autonomously replicating plasmid: cloning of the nicB gene of A. niger.
Gene - 2 Sept 1994
Verdoes J C, Punt P J, van der Berg P, Debets F, Stouthamer A H, van den Hondel C A
Abstract excerpt
The development of an improved gene cloning strategy by complementation of mutant alleles in Aspergillus niger is described. The strategy is based on the use of a fungal autonomously replicating vector, pAB4-ARp1. This vector was constructed by the introduction of a previously described sequence involved in autonomous replication (AMA1), into a pyrG integrative vector, pAB4-1. With vector pAB4-ARp1, a 10-100-fold...
Topics
- Aspergillus niger
- Cloning, Molecular
- Escherichia coli
- Genes, Fungal
- Genetic Complementation Test
- Genetic Vectors
- Phenotype
- Transformation, Bacterial
- Transformation, Genetic