Article
General vectors for archaeal hyperthermophiles: strategies based on a mobile intron and a plasmid.
FEMS microbiology reviews - 1 May 1996
Aagaard C, Leviev I, Aravalli R N, Forterre P, Prieur D, Garrett R A
Abstract excerpt
Although there are currently no cloning and expression vectors available for archaeal hyperthermophiles, small cryptic plasmids have been characterized for these organisms as well as viruses and introns capable of spreading between cells. Below, we review the recent progress in adapting these genetic elements as vectors for Pyrococcus furiosus and Sulfolobus acidocaldarius. An efficient and reliable...
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