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Article

New thermosensitive plasmid for gram-positive bacteria.

Journal of bacteriology - 1 Sept 1992

Maguin E, Duwat P, Hege T, Ehrlich D, Gruss A

Abstract excerpt

We isolated a replication-thermosensitive mutant of the broad-host-range replicon pWV01. The mutant pVE6002 is fully thermosensitive above 35 degrees C in both gram-negative and gram-positive bacteria. Four clustered mutations were identified in the gene encoding the replication protein of pVE6002. The thermosensitive derivative of the related plasmid pE194 carries a mutation in the analogous region but not in...

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Identifiers

PMID: 1324906
DOI: 10.1128/jb.174.17.5633-5638.1992
PMCID: PMC206509

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