Article
New thermosensitive plasmid for gram-positive bacteria.
Journal of bacteriology - 1 Sept 1992
Maguin E, Duwat P, Hege T, Ehrlich D, Gruss A
Abstract excerpt
We isolated a replication-thermosensitive mutant of the broad-host-range replicon pWV01. The mutant pVE6002 is fully thermosensitive above 35 degrees C in both gram-negative and gram-positive bacteria. Four clustered mutations were identified in the gene encoding the replication protein of pVE6002. The thermosensitive derivative of the related plasmid pE194 carries a mutation in the analogous region but not in...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- DNA Helicases
- DNA Replication
- DNA Transposable Elements
- DNA, Bacterial
- DNA-Binding Proteins
- Gram-Positive Bacteria
- Molecular Sequence Data
- Mutation