Creating a Library of Random Transposon Mutants in Leptospira.

Abstract excerpt

Generation of a random transposon mutant library is advantageous in Leptospira as site-directed mutagenesis remains a challenge, especially in pathogenic species. This procedure is typically completed by transformation of Leptospira with a Himar1-containing plasmid via conjugation with Escherichia coli as a donor cell. Here, we describe the methodology to generate random transposon mutants in the saprophyte...

Topics

• Leptospira
• DNA Transposable Elements
• Gene Library
• Plasmids
• Escherichia coli
• Mutagenesis, Insertional
• Mutation