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Article

Transposon mutagenesis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Kulasekara Hemantha D

Abstract excerpt

Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we present a detailed protocol for using Himar1 mariner-based...

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Share to DNA Transposable Elements
Share to Genetic Vectors
Share to Mutagenesis, Insertional

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 24818929
DOI: 10.1007/978-1-4939-0473-0_39

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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