Article
Screening of a Leptospira biflexa mutant library to identify genes involved in ethidium bromide tolerance.
Applied and environmental microbiology - 1 Oct 2014
Pětrošová Helena, Picardeau Mathieu
Abstract excerpt
Leptospira spp. are spirochete bacteria comprising both pathogenic and free-living species. The saprophyte L. biflexa is a model bacterium for studying leptospiral biology due to relative ease of culturing and genetic manipulation. In this study, we constructed a library of 4,996 random transposon mutants in L. biflexa. We screened the library for increased susceptibility to the DNA intercalating agent, ethidium...
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