Article
Creating a Library of Random Transposon Mutants in Leptospira.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Pappas Christopher J, Xu Hui, Motaleb Md A
Abstract excerpt
Generation of a random transposon mutant library is advantageous in Leptospira as site-directed mutagenesis remains a challenge, especially in pathogenic species. This procedure is typically completed by transformation of Leptospira with a Himar1 containing plasmid via conjugation with Escherichia coli as a donor cell. Here we describe the methodology to generate random transposon mutants in the saprophyte...
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