Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Transposon Sequencing in Leptospira interrogans.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026

Matsunaga James

Abstract excerpt

The development of random transposon mutagenesis in L. interrogans has enabled the creation of large libraries of mutants, facilitating the identification of genes involved in key phenotypes such as virulence. However, phenotypic screening of individual mutants from these libraries remains time-consuming and labor-intensive. Here, we describe a transposon-sequencing protocol (Tn-Seq) that combines random...

Read the complete abstract on PubMed

Topics

DNA Transposable Elements
High-Throughput Nucleotide Sequencing
Leptospira interrogans
Animals
Mutagenesis, Insertional
Virulence
Gene Library

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Mutation

Virulence Factors

Source

Identifiers

PMID: 42681487
DOI: 10.1007/978-1-0716-5500-9_11

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 25 Oct 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Transposon Sequencing in Leptospira interrogans.PMID 42681487Read ↗

Transposon Sequencing in Leptospira interrogans.Semantic proximity Read ↗

Construction of a library of random mutants in the spirochete Leptospira biflexa using a mariner transposon.Semantic proximity Read ↗

Random Insertional Mutagenesis of<i>Leptospira interrogans</i>, the Agent of Leptospirosis, Using a<i>mariner</i>TransposonSemantic proximity Read ↗

Transposon mutagenesis as an approach to improved understanding of Borrelia pathogenesis and biologySemantic proximity Read ↗

Transposon-insertion sequencing in a clinical isolate of <i>Legionella pneumophila</i> identifies essential genes and determinants of natural transformationSemantic proximity Read ↗

Creating a Library of Random Transposon Mutants in Leptospira.Semantic proximity Read ↗

Dual transposon sequencing (Dual Tn-seq) to probe genome-wide genetic interactionsSemantic proximity Read ↗

A group B Streptococcus indexed transposon mutant library to accelerate genetic research on an important perinatal pathogen.Semantic proximity Read ↗

Implementation and Data Analysis of Tn-seq, Whole-Genome Resequencing, and Single-Molecule Real-Time Sequencing for Bacterial Genetics.Semantic proximity Read ↗

Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung.Semantic proximity Read ↗

Genome-Wide Mutagenesis in Borrelia burgdorferi.Semantic proximity Read ↗

Creating a Library of Random Transposon Mutants in Leptospira.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.