Article
Construction of a library of random mutants in the spirochete Leptospira biflexa using a mariner transposon.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Slamti Leyla, Picardeau Mathieu
Abstract excerpt
In comparison to other bacterial species, genetics of leptospires are in their infancy. Recently, we developed a system for random transposon mutagenesis in the saprophyte Leptospira biflexa and then applied this approach to the pathogen L. interrogans. Thousands of random mutants can be readily obtained in -L. -biflexa by random insertion of Himar1 in the genome, thereby generating extensive libraries of mutants...
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