Article
Construction of Rhodococcus random mutagenesis libraries using Tn5 transposition complexes.
Microbiology (Reading, England) - 1 Sept 2001
Fernandes Paula J, Powell Justin A C, Archer John A C
Abstract excerpt
The ability to generate tagged mutants of Rhodococcus spp. will facilitate a deeper understanding of this medically and commercially important genus. The absence of efficient transposon systems in these organisms has here been overcome by the use of Tn5-based DNA-protein transposition complexes which can transpose at high efficiency. To achieve this, electroporation efficiencies and antibiotic selection were...
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