Article
A system for transposon mutagenesis of Bartonella bacilliformis.
Journal of microbiological methods - 1 Dec 2022
Andrew Finley J, Hicks Linda D, Minnick Michael F
Abstract excerpt
Bartonella bacilliformis is the etiologic agent of Carrión's disease in South America. Lack of a system for random mutagenesis has significantly hampered research on the pathogen's molecular biology. Here, we describe a transposon (Tn)-based mutagenesis strategy for B. bacilliformis using pSAM_Rl; a Tn-mariner delivery vector originally constructed for members of the Rhizobiaceae family. Following electroporation...
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