Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing.

doi:10.1038/s41588-026-02675-y

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Article

Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing.

Nature genetics - 1 Sept 2026

Price Daniel, Zemlyanskiy Grigory, Trakarnphornsombat Watanya, Forne Ignasi, Volkova Nadezda, Dubusse Louis, Cooper Alexandra J, Imhof Axel, Radzisheuskaya Aliaksandra

Abstract excerpt

Histone post-translational modifications are fundamental to genome regulation, yet dissecting the functions of individual histone marks in mammals remains challenging due to the presence of multiple histone gene copies. Here we develop a high-throughput clustered regularly interspaced short palindromic repeats (CRISPR) prime editing platform enabling precise, reversible and combinatorial mutagenesis of canonical...

Read the complete abstract on PubMed

Topics

Histones
Animals
Lysine
Mice
Gene Editing
CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Protein Processing, Post-Translational

Mouse Embryonic Stem Cells

Humans

Clustered Regularly Interspaced Short Palindromic Repeats

Mutation

Genomic Instability

Saccharomyces cerevisiae

Source

Open in PubMed Open full text

Identifiers

PMID: 42420522
DOI: 10.1038/s41588-026-02675-y
PMCID: PMC13553292

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