Article
A histone H2A and H2B point mutant library defines essential in vivo functions in Drosophila.
Cell reports - 23 Dec 2025
Sun Angyang, Wei Zilong, Zheng Jie, Zhang Xuedi, Gao Guanjun
Abstract excerpt
Histone variants and post-translational modifications are critical for chromatin organization and gene regulation, yet the in vivo, residue-specific functions of histone H2A and H2B in metazoans remain poorly understood. Here, we present a Drosophila histone H2A and H2B point mutant library, generated using a φC31 integrase-based system to replace the endogenous histone gene cluster with engineered mutant arrays....
Read the complete abstract on PubMedTopics
- Animals
- Histones
- Drosophila Proteins
- Drosophila melanogaster
- Gene Library
- Mutation
- Chromatin