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Article

Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes.

Science (New York, N.Y.) - 11 Oct 2024

Chen Xi Dawn, Chen Zeyu, Wythes George, Zhang Yifan, Orr Benno C, Sun Gary, Chao Yu-Kai, Navarro Torres Andrea, Thao Ka, Vallurupalli Mounica, Sun Jing, Borji Mehdi, Tkacik Emre, Chen Haiqi, Bernstein Bradley E, Chen Fei

Abstract excerpt

Deciphering the context-specific relationship between sequence and function is a major challenge in genomics. Existing tools for inducing locus-specific hypermutation and evolution in the native genome context are limited. Here we present a programmable platform for long-range, locus-specific hypermutation called helicase-assisted continuous editing (HACE). HACE leverages CRISPR-Cas9 to target a processive...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39388570
DOI: 10.1126/science.adn5876
PMCID: PMC13492663

Genes

CD69
SF3B1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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