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Article

Biolayer Interferometry for Investigating Membrane Protein-Inhibitor Binding: TACAN Mutant and GsMTx4 As a Model System.

Journal of visualized experiments : JoVE - 8 May 2026

Mazher Mayada, Michell Belinda, Rouiller Isabelle

Abstract excerpt

Bio-Layer Interferometry (BLI) is an efficient biophysical technique that enables real-time, quantitative analysis of biomolecular interactions. Although commonly used for soluble proteins, their application to membrane proteins remains challenging due to hydrophobicity and detergent dependence. This study presents an optimized BLI protocol for characterizing interactions between detergent-solubilized membrane...

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Identifiers

PMID: 42184262
DOI: 10.3791/69348

Genes

TMEM120A

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 10 Sept 2026.

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