Article

Single-molecule Dynamic In-Solution Inhibition Assay: A Method for Full Kinetic Profiling of Drug Candidate Binding to GPCRs in Native Membranes

2021-09-16

Abstract excerpt

Kinetic profiling of drug–target interactions using surface-based label-free technologies is well established for water-soluble pharmaceutical targets but is difficult to execute for membrane proteins in general and G-protein–coupled receptors (GPCRs) in particular. That is because surface immobilization of GPCRs tends to alter their configuration and function, leading to low target coverage and non-specific bindi...

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Literature Corpus work: 92291bfe-3ede-5efb-80e8-2ca8a74676e6
DOI: 10.1101/2021.09.16.460640

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