Article
SDS-CRISPR for Single-Nucleotide Variant Detection.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) - 1 Jun 2026
Guan Xin, Guo Chong, Zhang Jiongyu, Yang Rui, Ramachandra Yerramsetti, Hou Chengyu, Pei Minjie, Zhang Shuo, Schalper Kurt T, Liu Xingye, Wu Qian, Bulsara Ketan R, Liu Changchun
Abstract excerpt
The CRISPR-Cas12a system offers a promising platform for simple and sensitive nucleic acid diagnostics, including tumor-associated variant detection and infectious agent identification. However, its intrinsic mismatch tolerance limits its ability to accurately detect single-nucleotide variants (S...
Topics
- Humans
- Polymorphism, Single Nucleotide
- CRISPR-Cas Systems
- Glioma
- Isocitrate Dehydrogenase
- High-Throughput Nucleotide Sequencing
- Mutation
- Clustered Regularly Interspaced Short Palindromic Repeats