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Article

SPARC: An Orthogonal Cas12a/Cas13a Dual-Channel CRISPR Platform for Reliable SNV Identification and Mutation Confirmation.

Analytical chemistry - 15 Jul 2025

Zhong Jialing, Chen Yong, Dong Yueyu, Guo Chuanghao, Liu Yizhen

Abstract excerpt

Rapid and reliable detection of single nucleotide variants (SNVs) is essential for accurate pathogen diagnostics, genetic mutation screening, and personalized medicine. However, existing CRISPR-based nucleic acid detection platforms frequently suffer from ambiguous signal interpretation, specificity limitations, and complex assay workflows. Herein, we introduce SPARC (specific and precise mutation recognition...

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PMID: 40619644
DOI: 10.1021/acs.analchem.5c02141

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SPARC

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