Probing the Specificity of Fluorescent Deoxyribozymes Using Single-Step Selections and Machine Learning.

Abstract excerpt

The ability of proteins and nucleic acids to form specific binding sites for ligands is critical for biological function, and methods to modulate biochemical specificity are important for fields such as enzyme engineering and drug design. Here, we systematically investigated the specificities of self-phosphorylating deoxyribozymes that convert the coumarin substrate 4-MUP into a fluorescent product using...

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