In vitro selection of an allosteric ribozyme that transduces analytes to amplicons.

doi:10.1038/5236

Back to search

Article

In vitro selection of an allosteric ribozyme that transduces analytes to amplicons.

Nature biotechnology - 1 Jan 1999

Robertson M P, Ellington A D

Abstract excerpt

We have selected an allosteric ribozyme ligase from a random sequence population that is activated up to 10,000-fold by oligonucleotide effectors. The ribozyme conforms to a classic two-state model for allostery in which the equilibrium between inactive and active conformers is dramatically alter...

Topics

Allosteric Site
Base Sequence
DNA Primers
Gene Amplification
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
RNA Ligase (ATP)
RNA, Catalytic
Reverse Transcriptase Polymerase Chain Reaction
Selection, Genetic
Substrate Specificity

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.