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Article

Systematic exploration of active site mutations on human deoxycytidine kinase substrate specificity.

Biochemistry - 22 Apr 2008

Iyidogan Pinar, Lutz Stefan

Abstract excerpt

Human deoxycytidine kinase (dCK) is responsible for the phosphorylation of a number of clinically important nucleoside analogue prodrugs in addition to its natural substrates, 2'-deoxycytidine, 2'-deoxyguanosine, and 2'-deoxyadenosine. To improve the low catalytic activity and tailor the substrate specificity of dCK, we have constructed libraries of mutant enzymes and tested them for thymidine kinase (tk)...

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Source

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Identifiers

PMID: 18361501
DOI: 10.1021/bi800157e
PMCID: PMC2736870

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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