Article
A class I ligase ribozyme with reduced Mg2+ dependence: Selection, sequence analysis, and identification of functional tertiary interactions.
RNA (New York, N.Y.) - 1 Dec 2009
Bagby Sarah C, Bergman Nicholas H, Shechner David M, Yen Catherine, Bartel David P
Abstract excerpt
The class I ligase was among the first ribozymes to have been isolated from random sequences and represents the catalytic core of several RNA-directed RNA polymerase ribozymes. The ligase is also notable for its catalytic efficiency and structural complexity. Here, we report an improved version of this ribozyme, arising from selection that targeted the kinetics of the chemical step. Compared with the parent...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.