Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A recurrent sequencing artifact on Illumina sequencers with two-color fluorescent dye chemistry and its impact on somatic variant detection.

Genome biology - 17 Apr 2026

Fu Beverly J, Viswanadham Vinayak V, Maziec Dominika, Jin Hu, Park Peter J

Abstract excerpt

BACKGROUND: The sequencing-by-synthesis technology by Illumina, Inc. enables efficient and scalable readouts of mutations from genomic data. To enhance sequencing speed and efficiency, Illumina has shifted from the four-color base calling chemistry of the HiSeq series to a two-color fluorescent dye chemistry in the NovaSeq series. Benchmarking sequencing artifacts due to biases in the newer chemistry is important...

Topics

Join the communities discussing this publication.

Artifacts
Fluorescent Dyes
Gene Frequency
TG gene
High-Throughput Nucleotide Sequencing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sequence Analysis, DNA

Whole Genome Sequencing

Source

Open in PubMed Open full text

Identifiers

PMID: 41998753
DOI: 10.1186/s13059-026-04081-3
PMCID: PMC13218037

Genes

TG

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A recurrent sequencing artifact on Illumina sequencers with two-color fluorescent dye chemistry and its impact on somatic variant detection.PMID 41998753Read ↗

A recurrent sequencing artifact on Illumina sequencers with two-color fluorescent dye chemistry and its impact on somatic variant detectionSemantic proximity Read ↗

Systematic artifacts from Illumina two-color chemistry confound variant identification and actionability in clinical panelsSemantic proximity Read ↗

DNA damage is a major cause of sequencing errors, directly confounding variant identificationSemantic proximity Read ↗

Next-generation sequencing methodologies to detect low-frequency mutations: "Catch me if you can".Semantic proximity Read ↗

Benchmarking of duplex sequencing approaches to reveal somatic mutation landscapesSemantic proximity Read ↗

Detection of low-frequency mutations and removal of heat-induced artifactual mutations using Duplex SequencingSemantic proximity Read ↗

High-frequency, low-coverage "false positives" mutations may be true in GS Junior sequencing studies.Semantic proximity Read ↗

Conpair: concordance and contamination estimator for matched tumor–normal pairsSemantic proximity Read ↗

Chasing Sequencing Perfection: Marching Toward Higher Accuracy and Lower Costs.Semantic proximity Read ↗

A robust benchmark for detecting low-frequency variants in the HG002 Genome In A Bottle NIST reference material.Semantic proximity Read ↗

pyAmpli: an amplicon-based variant filter pipeline for targeted resequencing data.Semantic proximity Read ↗

MERIT: a Mutation Error Rate Identification Toolkit for Ultra-deep Sequencing ApplicationsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.