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Article

pyAmpli: an amplicon-based variant filter pipeline for targeted resequencing data.

BMC bioinformatics - 14 Dec 2017

Beyens Matthias, Boeckx Nele, Van Camp Guy, Op de Beeck Ken, Vandeweyer Geert

Abstract excerpt

BACKGROUND: Haloplex targeted resequencing is a popular method to analyze both germline and somatic variants in gene panels. However, involved wet-lab procedures may introduce false positives that need to be considered in subsequent data-analysis. No variant filtering rationale addressing amplicon enrichment related systematic errors, in the form of an all-in-one package, exists to our knowledge. RESULTS: We...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29237398
DOI: 10.1186/s12859-017-1985-1
PMCID: PMC5729461

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