Article

Systematic artifacts from Illumina two-color chemistry confound variant identification and actionability in clinical panels

2025-10-20

Abstract excerpt

Illumina short-read sequencing underpins clinical cancer genomics, with targeted panels widely used to detect actionable variants. Newer Illumina platforms employ a two-color chemistry to accelerate sequencing, but its impact on variant identification has not been systematically evaluated. Here we show that two-color platforms generate recurrent T>G artifacts in targeted panels at low variant allele fractions, pre...

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Literature Corpus work: f88e7f92-671f-520b-acfe-5842317bfc59
DOI: 10.1101/2025.10.17.25338254

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