Article

A recurrent sequencing artifact on Illumina sequencers with two-color fluorescent dye chemistry and its impact on somatic variant detection

2025-09-29

Abstract excerpt

<h4>Background</h4> The sequencing-by-synthesis technology by Illumina, Inc. enables efficient and scalable readouts of mutations from genomic data. To enhance sequencing speed and efficiency, Illumina has shifted from the four-color base calling chemistry of the HiSeq series to a two-color fluorescent dye chemistry in the NovaSeq series. Benchmarking sequencing artifacts due to biases in the newer chemistry is i...

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Literature Corpus work: 1244163c-25fb-578f-b3f0-5ff24673c2cc
DOI: 10.1101/2025.09.27.678978

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