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Article

High-frequency, low-coverage "false positives" mutations may be true in GS Junior sequencing studies.

Scientific reports - 23 Oct 2017

Yang Zhiliang, Sun Guilian

Abstract excerpt

The GS Junior sequencer provides simplified procedures for library preparation and data processing. Errors in pyrosequencing generate some biases during library construction and emulsion PCR amplification. False-positive mutations are identified by related characteristics described in the manufacturer's manual, and some detected mutations may have 'borderline' characteristics when they are detected in few reads...

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Identifiers

PMID: 29062110
DOI: 10.1038/s41598-017-13116-6
PMCID: PMC5653793

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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