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Article

Genotype identification of Math1/LacZ knockout mice based on real-time PCR with SYBR Green I dye.

Journal of neuroscience methods - 30 Jul 2004

Krizhanovsky Valery, Golenser Esther, Ben-Arie Nissim

Abstract excerpt

Knockout mice are widely used in all fields of biomedical research. Determining the genotype of every newborn mouse is a tedious task, usually performed by Southern blot hybridization or Polymerase Chain Reaction (PCR). We describe here a quick and simple genotype identification assay based on real-time PCR and SYBR Green I dye, without using fluorescent primers. The discrimination between the wild type and...

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PMID: 15183270
DOI: 10.1016/j.jneumeth.2004.01.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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