Article
Efficient detection, quantification and enrichment of subtle allelic alterations.
DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Oct 2012
Chen Jianbin, Zhang Xi, Wang Tiansu, Li Zhendong, Guan Guijun, Hong Yunhan
Abstract excerpt
Gene targeting (GT) can introduce subtle alterations into a particular locus and represents a powerful tool for genome editing. Engineered zinc finger nucleases (ZFNs) are effective for generating minor allelic alterations. Efficient detection of such minor alterations remains one of the challenges in ZFN-mediated GT experiments. Here, we report the establishment of procedures allowing for efficient detection,...
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