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Single-tube Ptprc SNP genotyping of JAXBoy (CD45.1) and C57BL/6J (CD45.2) mice by endpoint PCR and gel electrophoresis.

Molecular and cellular probes - 1 Jun 2024

Ryan Claire E, Salvetti Thomas R, Baum Ilana R, Figueroa Brandon A, LeBere Brittany E, Alberti Michael O

Abstract excerpt

Allelic variation at the Ptprc gene, which encodes the pan-leukocyte marker CD45/Ly5, is commonly exploited to track hematopoietic reconstitution by flow cytometry in mixed bone marrow chimera transplant experiments. Historically, this was accomplished using bone marrow from C57BL/6 (Ptprcb/CD45.2/Ly5.2) and congenic B6.SJL-PtprcaPepcb/Boy (Ptprca/CD45.1/Ly5.1) mice. Recently, the Jackson Laboratory directly...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 38697553
DOI: 10.1016/j.mcp.2024.101962
PMCID: PMC11146669

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