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Article

Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation.

PloS one - 1 Jan 2026

Sawitzke James A, Costantino Nina, Caballero Adriana Castillo, Hutchinson Ellen, Barenghi Alessandro, Thomason Lynn C, Court Donald L

Abstract excerpt

Assembly of intact, replicating plasmids from linear DNA fragments introduced into bacterial cells, i.e., in vivo cloning, is a facile genetic engineering technology that avoids many of the problems associated with standard in vitro cloning. Here, we report characterization of various parameters of in vivo linear DNA assembly mediated by either the RecET recombination system or the bacteriophage λ Red...

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PMID: 41931560
DOI: 10.1371/journal.pone.0344368
PMCID: PMC13048471

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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