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Article

Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants.

Proceedings of the National Academy of Sciences of the United States of America - 23 Dec 2003

Costantino Nina, Court Donald L

Abstract excerpt

Homologous recombination can be used to generate recombinants on episomes or directly on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA (ssDNA) oligonucleotides (oligos). Such recombination is possible because bacteriophage lambda-encoded functions, called Red, efficiently recombine linear DNA with homologies as short as 20-70 bases. This technology, termed recombineering,...

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Identifiers

PMID: 14673109
DOI: 10.1073/pnas.2434959100
PMCID: PMC307639

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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