Article
A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation.
Nucleic acids research - 1 Jan 2014
Carballeira José Daniel, González-Pérez Blanca, Moncalián Gabriel, de la Cruz Fernando
Abstract excerpt
Relaxases act as DNA selection sieves in conjugative plasmid transfer. Most plasmid relaxases belong to the HUH endonuclease family. TrwC, the relaxase of plasmid R388, is the prototype of the HUH relaxase family, which also includes TraI of plasmid F. In this article we demonstrate that TrwC processes its target nic-site by means of a highly secure double lock and key mechanism. It is controlled both by TrwC-DNA...
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