Article
Ordered deletions for DNA sequencing and in vitro mutagenesis by polymerase extension and exonuclease III gapping of circular templates.
Nucleic acids research - 25 May 1990
Henikoff S
Abstract excerpt
A simple method is described for generating nested deletions from any fixed point in a cloned inset. Starting with a single-stranded phagemid template, T4 DNA polymerase is used to extend an annealed primer. This leads to a fully double-stranded circular molecule with a nick or small gap just 5' to the primer. Exonuclease III initiates progressive digestion from the resulting 3' end. Removal of timed aliquots and...
Topics
- Animals
- Base Sequence
- DNA
- DNA, Circular
- DNA-Directed DNA Polymerase
- Drosophila
- Exodeoxyribonucleases
- Genetic Techniques
- Molecular Sequence Data
- Mutation
- T-Phages