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Article

Ordered deletions for DNA sequencing and in vitro mutagenesis by polymerase extension and exonuclease III gapping of circular templates.

Nucleic acids research - 25 May 1990

Henikoff S

Abstract excerpt

A simple method is described for generating nested deletions from any fixed point in a cloned inset. Starting with a single-stranded phagemid template, T4 DNA polymerase is used to extend an annealed primer. This leads to a fully double-stranded circular molecule with a nick or small gap just 5' to the primer. Exonuclease III initiates progressive digestion from the resulting 3' end. Removal of timed aliquots and...

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Identifiers

PMID: 2190184
DOI: 10.1093/nar/18.10.2961
PMCID: PMC330825

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