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Rapid and reliable DNA assembly via ligase cycling reaction.

ACS synthetic biology - 21 Feb 2014

de Kok Stefan, Stanton Leslie H, Slaby Todd, Durot Maxime, Holmes Victor F, Patel Kedar G, Platt Darren, Shapland Elaine B, Serber Zach, Dean Jed, Newman Jack D, Chandran Sunil S

Abstract excerpt

Assembly of DNA parts into DNA constructs is a foundational technology in the emerging field of synthetic biology. An efficient DNA assembly method is particularly important for high-throughput, automated DNA assembly in biofabrication facilities and therefore we investigated one-step, scarless DNA assembly via ligase cycling reaction (LCR). LCR assembly uses single-stranded bridging oligos complementary to the...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 24932563
DOI: 10.1021/sb4001992

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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