Article
Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate.
Genetics - 1 Nov 2010
Mosberg J A, Lajoie M J, Church G M
Abstract excerpt
The phage lambda-derived Red recombination system is a powerful tool for making targeted genetic changes in Escherichia coli, providing a simple and versatile method for generating insertion, deletion, and point mutations on chromosomal, plasmid, or BAC targets. However, despite the common use of this system, the detailed mechanism by which lambda Red mediates double-stranded DNA recombination remains uncertain....
Topics
- Alleles
- Bacteriophage lambda
- Base Pair Mismatch
- Chromosome Segregation
- DNA, Bacterial
- DNA, Single-Stranded
- Escherichia coli
- Genetic Engineering
- Mutation
- Phosphorothioate Oligonucleotides
- Recombination, Genetic