Oligo-Mediated Recombineering and its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis.

doi:10.1007/978-1-0716-1460-0_14

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Article

Oligo-Mediated Recombineering and its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Murphy Kenan C

Abstract excerpt

Phage recombination systems have been instrumental in the development of gene modification technologies for bacterial pathogens. In particular, the Che9 phage RecET system has been used successfully for over 10 years for making gene knockouts and fusions in Mycobacterium tuberculosis. This "recombineering" technology typically uses linear dsDNA substrates that contain a drug-resistance marker flanked by (up to)...

Topics

Chromosomes, Bacterial
Gene Deletion
Gene Editing
Gene Fusion
Genetic Engineering
Genome, Bacterial
Mutagenesis, Insertional
Mycobacterium tuberculosis
Oligonucleotides
Polymorphism, Single Nucleotide
Recombination, Genetic

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PMID: 34235660
DOI: 10.1007/978-1-0716-1460-0_14

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