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Article

Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases.

PloS one - 1 Jan 2012

Mosberg Joshua A, Gregg Christopher J, Lajoie Marc J, Wang Harris H, Church George M

Abstract excerpt

Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous nucleases may hinder recombination by degrading the exogenous DNA used for recombineering. In this work, we identify ExoVII as a nuclease which degrades the ends of single-stranded DNA (ssDNA)...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA, Single-Stranded

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Identifiers

PMID: 22957093
DOI: 10.1371/journal.pone.0044638
PMCID: PMC3434165

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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