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Article

Semi-rational design of psychrophilic cellobiose 2-epimerase for improved substrate affinity and enhanced low-temperature epimerization activity.

Bioresource technology - 1 Jun 2026

Xu Hu, Lv Niannian, Liu Yongqin

Abstract excerpt

Psychrophilic cellobiose 2-epimerase (CE) is a promising biocatalyst for cold-process dairy manufacturing, yet its industrial application is hampered by the inherent trade-off between low-temperature activity and substrate affinity. To overcome this limitation, a semi-rational design strategy was implemented on a psychrophilic CE (psyCE). A GFP-based fluorescence assay was developed to normalize mutant...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41802478
DOI: 10.1016/j.biortech.2026.134363

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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