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Structure-Guided Engineering Unveils Deeper Substrate Channel in Processive Endoglucanase EG5C-1 Contributing to Enhanced Catalytic Efficiency and Processivity.

ACS synthetic biology - 20 Dec 2024

Wang Jialing, Chen Jie, Lv Kemin, Gao Zhen, Li Jiahuang, Wu Bin, He Bingfang, Schenk Gerhard

Abstract excerpt

Processive endoglucanases have generated significant interest due to their bifunctionality in the degradation of cellulose and low product inhibition. However, enhancing their catalytic efficiency through engineering remains a formidable challenge. To address this bottleneck, our engineering efforts targeted loop regions located in the substrate channel of processive endoglucanase EG5C-1. Guided by a comparative...

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Cellulase
Cellulose
Molecular Dynamics Simulation
Mutation
Protein Engineering

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PMID: 39579149
DOI: 10.1021/acssynbio.4c00562

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