Semi-rational design and molecular dynamics simulations study of the thermostability enhancement of cellobiose 2-epimerases.

Abstract excerpt

Directed evolution using random mutation in vast sequence space leads to the low probability of obtaining target proteins. Emerging engineering strategies with computational tools are developed for more trustable outcomes. We used some semi-rational design methods to modify an industrial enzyme, namely cellobiose 2-epimerase (CE). A mutant was selected for its better thermostability and isomerization activity....

Topics

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• Carbohydrate Epimerases
• Enzyme Stability
• Kinetics
• Molecular Dynamics Simulation
• Mutation