Balancing α6/α6 barrel kinetic stability and loop flexibility in a thermophilic cellobiose 2-epimerase enhanced lower temperature lactulose biosynthesis.

Abstract excerpt

Cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus (CsCE) is a biocatalyst for lactulose synthesis effectively at 75 °C. However, its industrial usefulness is limited by high thermal requirements needed to meet such a high optimum temperature. From structural studies, we hypothesized that the enzyme's activity and lower temperature adaptability are governed by two structural elements; the α6/α6...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Carbohydrate Epimerases
• Share to Computer Simulation