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Engineering a bifunctional cellobiose 2-epimerase into an isomerase capable of selective lactulose production.

Bioresource technology - 1 May 2026

Toplak Ana, Wijma Hein J, Janssen Christina J, Houben Lars, Altay Meniz, Halmschlag Birthe S, Janssen Dick B, Cabri Walter, Ricci Antonio

Abstract excerpt

Lactulose is listed by the World Health Organization as an essential medicine and is also a prebiotic. Its industrial production from cheap and abundant lactose suffers from low yields and expensive purification procedures. Therefore, cellobiose 2-epimerases have received attention as they can catalyze the required isomerization of lactose to lactulose. The main challenge with these enzymes is that they are much...

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PMID: 41707773
DOI: 10.1016/j.biortech.2026.134241

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