Enhancing one-step d-tagatose production from d-fructose through combinatorial structure-guided and evolution-based engineering of tagatose 4-epimerase.

Abstract excerpt

d-tagatose is a highly valuable rare sugar with significant application potential. However, its widespread use is severely limited by high production costs. One-step conversion from d-fructose represents an economically attractive bioprocess; nevertheless, the low catalytic efficiency of natural tagatose 4-epimerases (T4Es) toward d-fructose remains a major bottleneck for industrial application. Therefore,...

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